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Assemble RNA-Seq data like so:


 Trinity --seqType fq --left reads_1.fq --right reads_2.fq --CPU 6 --max_memory 20G 

 

 

Installation

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module load library/atlas/3.10.2

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Build Trinity by typing 'make' in the base installation directory.

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cd /sw/bioinformatics/trinity/2.2.0

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make

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~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

 

Performing Unit Tests of Build

 

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

JellyFish:               has been Installed Properly

Inchworm:                has been Installed Properly

Chrysalis:               has been Installed Properly

QuantifyGraph:           has been Installed Properly

GraphFromFasta:          has been Installed Properly

ReadsToTranscripts:      has been Installed Properly

fastool:                 has been Installed Properly

parafly:                 has been Installed Properly

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~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


Performing Unit Tests of Build
 
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
JellyFish:               has been Installed Properly
Inchworm:                has been Installed Properly
Chrysalis:               has been Installed Properly
QuantifyGraph:           has been Installed Properly
GraphFromFasta:          has been Installed Properly
ReadsToTranscripts:      has been Installed Properly
fastool:                 has been Installed Properly
parafly:                 has been Installed Properly
samtools-0.1.19          has been Installed Properly

 


Reference

 

  1. https://github.com/trinityrnaseq/trinityrnaseq/releases
  2. https://github.com/trinityrnaseq/trinityrnaseq/wiki