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Assemble RNA-Seq data like so:


 Trinity --seqType fq --left reads_1.fq --right reads_2.fq --CPU 6 --max_memory 20G 

 

 

Installation

module load library/atlas/3.10.2

Build Trinity by typing 'make' in the base installation directory.

 

cd /sw/bioinformatics/trinity/2.2.0

make

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

 

Performing Unit Tests of Build

 

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

JellyFish:               has been Installed Properly

Inchworm:                has been Installed Properly

Chrysalis:               has been Installed Properly

QuantifyGraph:           has been Installed Properly

GraphFromFasta:          has been Installed Properly

ReadsToTranscripts:      has been Installed Properly

fastool:                 has been Installed Properly

parafly:                 has been Installed Properly

samtools-0.1.19          has been Installed Properly


Reference

 

  1. https://github.com/trinityrnaseq/trinityrnaseq/releases
  2. https://github.com/trinityrnaseq/trinityrnaseq/wiki