Skip to end of metadata
Go to start of metadata

You are viewing an old version of this page. View the current version.

Compare with Current View Page History

Version 1 Current »

Introduction

http://www.repeatmasker.org/
RepeatMasker is a program that screens DNA sequences for interspersed repeats and low complexity DNA sequences. The output of the program is a detailed annotation of the repeats that are present in the query sequence as well as a modified version of the query sequence in which all the annotated repeats have been masked (default: replaced by Ns). On average, almost 50% of a human genomic DNA sequence currently will be masked by the program. Sequence comparisons in RepeatMasker are performed by one of several popular search engines including, cross_match, ABBlast/WUBlast, RMBlast and Decypher.

Usage

module load bioinformatics/repeatmasker/3.3.0-p1

Installation

mkdir -p /sw/bioinformatics/repeatmasker/3.3.0-p1/SRC
tar -zxvf RepeatMasker-open-3-3-0-p1.tar.gz
cd /sw/bioinformatics/repeatmasker/3.3.0-p1/SRC/RepeatMasker
mv * /sw/bioinformatics/repeatmasker/3.3.0-p1/
cd /sw/bioinformatics/repeatmasker/3.3.0-p1/
perl ./configure


 perl ./configure
RepeatMasker Configuration Program
This program assists with the configuration of the
RepeatMasker program.  The next set of screens will ask
you to enter information pertaining to your system
configuration.  At the end of the program your RepeatMasker
installation will be ready to use.
<PRESS ENTER TO CONTINUE>
**PERL INSTALLATION PATH**

  This is the full path to the Perl interpreter.
  e.g. /usr/local/bin/perl or enter "env" if you prefer to use
  the "/usr/bin/env perl" mechanism to locate perl.

Enter path [ /usr/bin/perl ]:
**REPEATMASKER INSTALLATION PATH**

  This is the path to the location where
  the RepeatMasker program has been installed.

Enter path [ /sw/bioinformatics/repeatmasker/3.3.0-p1 ]:

  -- Building monolithic RM database...

**TRF INSTALLATION PATH**

  This is the path to the location where
  the TRF program can be found.  This is used by the RepeatProteinMask program.  [NOTE: This script assumes the trf program is called "trf".  If it is name trf###-linux.exe you will need to create a symlink called "trf" to it or rename it first.

Enter path [  ]: /sw/bioinformatics/repeatmasker/trf/4.04/bin

Add a Search Engine:
   1. CrossMatch: [ Un-configured ]
   2. RMBlast - NCBI Blast with RepeatMasker extensions: [ Un-configured ]
   3. WUBlast/ABBlast (required by DupMasker): [ Un-configured ]
   4. DeCypher (TimeLogic): [ Un-configured ]

   5. Done
Enter Selection: 2

**RMBlast (rmblastn) INSTALLATION PATH**

  This is the path to the location where
  the rmblastn and makeblastdb programs can be found.

Enter path [  ]: /sw/bioinformatics/repeatmasker/rmblast/2.2.23/bin
Building RMBlast frozen libraries..

Do you want RMBlast to be your default
search engine for Repeatmasker? (Y/N)  [ Y ]: Y

Add a Search Engine:
   1. CrossMatch: [ Un-configured ]
   2. RMBlast - NCBI Blast with RepeatMasker extensions: [ Configured, Default ]
   3. WUBlast/ABBlast (required by DupMasker): [ Un-configured ]
   4. DeCypher (TimeLogic): [ Un-configured ]

   5. Done

Enter Selection:5
Enter Selection: 5
 -- Setting perl interpreter...

Congratulations!  RepeatMasker is now ready to use.
The program is installed with a minimal repeat library
by default.  This library only contains simple, low-complexity,
and common artefact ( contaminate ) sequences.  These are
adequate for use with your own custom repeat library.  If you
plan to search using common species specific repeats you will
need to obtain the complete RepeatMasker repeat library from
GIRI ( www.giriinst.org ) and install it in /sw/bioinformatics/repeatmasker/3.3.0-p1.

Further documentation on the program may be found here:
  /sw/bioinformatics/repeatmasker/3.3.0-p1/repeatmasker.help

Complete RepeatMasker repeat Library

sign in and down load the libraries from : http://www.girinst.org/

cd /sw/bioinformatics/repeatmasker/3.3.0-p1/SRC
>>>>>>>>>
ls
RepBase14.11_REPET.embl.tar.gz  RepBase17.08.fasta.tar.gz  repeatmaskerlibraries-20090604.tar.gz  
RepBase17.08.embl.tar.gz        repeatmaskerlibraries-20120418.tar.gz
>>>>>>>>>>>>
tar -zxvf RepBase14.11_REPET.embl.tar.gz
tar -zxvf  RepBase17.08.embl.tar.gz
tar -zxvf RepBase17.08.fasta.tar.gz
tar -zxvf repeatmaskerlibraries-20090604.tar.gz
tar -zxvf  repeatmaskerlibraries-20120418.tar.gz

These are the repeat libraries for the program RepeatMasker. To
install, move or copy the file "RepeatMaskerLib.embl" into
the "Libraries" subdirectory of the RepeatMasker directory.
cd Libraries
cp -i RepeatMaskerLib.embl /sw/bioinformatics/repeatmasker/3.3.0-p1/Libraries
cd ../RepBase14.11_REPET.embl
cp -i *.fa /sw/bioinformatics/repeatmasker/3.3.0-p1/Libraries

Reference

  • No labels