Introduction
Trinity assembles transcript sequences from Illumina RNA-Seq data.
Usage
Assemble RNA-Seq data like so: Trinity --seqType fq --left reads_1.fq --right reads_2.fq --CPU 6 --max_memory 20G
Installation
module load library/atlas/3.10.2
Build Trinity by typing 'make' in the base installation directory.
cd /sw/bioinformatics/trinity/2.2.0
make
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Performing Unit Tests of Build
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
JellyFish: has been Installed Properly
Inchworm: has been Installed Properly
Chrysalis: has been Installed Properly
QuantifyGraph: has been Installed Properly
GraphFromFasta: has been Installed Properly
ReadsToTranscripts: has been Installed Properly
fastool: has been Installed Properly
parafly: has been Installed Properly
samtools-0.1.19 has been Installed Properly
Reference