Introduction

The preseq package is aimed at predicting and estimating the complexity of a genomic sequencing library, equivalent to predicting and estimating the number of redundant reads from a given sequencing depth and how many will be expected from additional sequencing using an initial sequencing experiment. The estimates can then be used to examine the utility of further sequencing, optimize the sequencing depth, or to screen multiple libraries to avoid low complexity samples.

Usage

module load  bioinformatics/preseq/preseq

Installation

module load gsl/gsl-1.15
cd /sw/bioinformatics/preseq/preseq
make all >makelog.txt 2>&1

Reference

1. http://smithlab.usc.edu/plone/software/librarycomplexity