SAMtools
Introduction
http://samtools.sourceforge.net/
http://sourceforge.net/projects/samtools/files/
SAM (Sequence Alignment/Map) format is a generic format for storing large nucleotide sequence alignments. SAM aims to be a format that:
Is flexible enough to store all the alignment information generated by various alignment programs;
Is simple enough to be easily generated by alignment programs or converted from existing alignment formats;
Is compact in file size;
Allows most of operations on the alignment to work on a stream without loading the whole alignment into memory;
Allows the file to be indexed by genomic position to efficiently retrieve all reads aligning to a locus.
Usage
module load bioinformatics/samtools/0.1.18
Installation
(SAMtools depends on the zlib library <http://www.zlib.net>. Version 1.2.3+ is preferred and with 1.2.3+ you can compile razip and use it to compress a FASTA file.) module load library/zlib/1.2.5 mkdir /sw/samtools/0.1.18 cd /sw/samtools/0.1.18 make make razip